Journal: Biological chemistry

Article Title: Mtr4 RNA helicase structures and interactions

doi: 10.1515/hsz-2020-0329

Figure Lengend Snippet: Structures of Mtr4 and Mtr4 Complexes

Article Snippet: We begin this review with a description of the overall Mtr4 architecture as established by the original structures, followed by an overview of the more recent structures and a discussion of mechanistic implications arising from these structures. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Protein PDB ID Protein RNA Nucleotide Method Resolution (Å) Year Mtr4 (without protein cofactors) sc Mtr4 4QU4 Mtr4 FL - - X-ray 3.4 2010 1,2 2XGJ Mtr4 Δ80 5-nt poly(A) * ADP 2.9 2010 3 nc FRH 4XGT # FRH Δ114 - - 3.1 2016 4 5DZR # FRH Δ114 - - 3.2 2016 4 5E02 # FRH Δ100 4-nt poly(A) † ADP 3.8 2016 4 6BB8 # FRH FL - - 3.5 2018 5 hs Mtr4 6IEG Mtr4 Δ70 - ATP 3.5 2019 6 Adaptor Complexes sc Mtr4 4U4C Mtr4 Δ80 Trf4 111–160 /Air2 1–62 - - X-ray 2.4 2014 7 5OOQ Mtr4 Δ80 , Nop53 58–91 - - 3.2 2017 8 hs Mtr4 6C90 Mtr4 Δ70, ΔArch ZCCHC8 659–707(CTD) - ADP 2.2 2018 9 6RO1 Mtr4 70–1042 , NVL 167–216 - ADP 3.1 2019 10 Inhibitor Complex hs Mtr4 6IEH Mtr4 Δ70 , NRDE2 163–266 - ATP X-ray 2.9 2019 6 Exosome Complexes sc Mtr4 4WFD Mtr4 1–20 , Rrp6 1–111 and Rrp47 1–103 - - X-ray 2.4 2014 11 6FSZ Mtr4 Δ80 , Exosome ‡ - - Cryo-EM 4.6 2018 12 6FT6 Mtr4 Δ80 Ribosome - 3.9 2018 12 hs Mtr4 6D6Q Mtr4 FL , Exosome § 62-nt DNA/RNA Hybrid AMPPNP 3.5 2018 13 6D6R Mtr4 FL , Exosome § 3.5 2018 13 Open in a separate window Details listed for protein, RNA, and nucleotide are original crystallization conditions unless otherwise stated.

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